Matt Painter will have two openings to fill on his staff.
Mike Jackson will resign effective April 30 from Purdue, according to a news release sent Wednesday.
Jackson, a first-year assistant for the Boilermakers, had been suspended since February in connection with an alcohol-related arrest. He pleaded guilty to a misdemeanor charge of operating a vehicle while intoxicated on March 25, and was sentenced to probation and 180 hours of community service, according to court records.
In a statement, Jackson said he enjoyed his time at Purdue and is "looking forward to the next chapter in my career."
Painter also has to replace Paul Lusk, who left to take the head coaching job at Missouri State.
Painter said after the team's banquet Monday that he thought Lusk's job had to be posted for a week, which would mean no announcements of a hire could be made until next Monday.
While Painter attended the Final Four in Houston last week, he said there was a lot of interest from coaches about Purdue's opening.